Coronavirus reverse genetic systems: Infectious clones and replicons
Identifieur interne : 001679 ( Main/Exploration ); précédent : 001678; suivant : 001680Coronavirus reverse genetic systems: Infectious clones and replicons
Auteurs : Fernando Almazán ; Isabel Sola ; Sonia Zu Iga ; Silvia Marquez-Jurado ; Lucia Morales ; Martina Becares ; Luis EnjuanesSource :
- Virus Research [ 0168-1702 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Coronavirus.
- Clones cellulaires, Génétique inverse, Réplicon, Virologie.
English descriptors
- KwdEn :
- MESH :
- genetics : Coronavirus.
- methods : Reverse Genetics, Virology.
- Clone Cells, Replicon.
Abstract
The four different reverse genetic systems developed for CoVs are reviewed and discussed. These systems were based on the use of BACs, in vitro ligation and vaccinia virus as a vector. CoV reverse genetics is an essential tool for the study of CoV replication, virus–host interaction and pathogenesis. These reverse genetic systems allow the development of genetically defined vaccines and the analysis of antiviral drugs.
Url:
DOI: 10.1016/j.virusres.2014.05.026
PubMed: 24930446
PubMed Central: 4727449
Affiliations:
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Becares</name></author><author><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24930446</idno><idno type="pmc">4727449</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727449</idno><idno type="RBID">PMC:4727449</idno><idno type="doi">10.1016/j.virusres.2014.05.026</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">001599</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001599</idno><idno type="wicri:Area/Pmc/Curation">001599</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001599</idno><idno type="wicri:Area/Pmc/Checkpoint">000912</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000912</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:24930446</idno><idno 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replicons</title><author><name sortKey="Almazan, Fernando" sort="Almazan, Fernando" uniqKey="Almazan F" first="Fernando" last="Almazán">Fernando Almazán</name></author><author><name sortKey="Sola, Isabel" sort="Sola, Isabel" uniqKey="Sola I" first="Isabel" last="Sola">Isabel Sola</name></author><author><name sortKey="Zu Iga, Sonia" sort="Zu Iga, Sonia" uniqKey="Zu Iga S" first="Sonia" last="Zu Iga">Sonia Zu Iga</name></author><author><name sortKey="Marquez Jurado, Silvia" sort="Marquez Jurado, Silvia" uniqKey="Marquez Jurado S" first="Silvia" last="Marquez-Jurado">Silvia Marquez-Jurado</name></author><author><name sortKey="Morales, Lucia" sort="Morales, Lucia" uniqKey="Morales L" first="Lucia" last="Morales">Lucia Morales</name></author><author><name sortKey="Becares, Martina" sort="Becares, Martina" uniqKey="Becares M" first="Martina" last="Becares">Martina Becares</name></author><author><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name></author></analytic><series><title level="j">Virus Research</title><idno type="ISSN">0168-1702</idno><idno type="eISSN">1872-7492</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Clone Cells</term><term>Coronavirus (genetics)</term><term>Replicon</term><term>Reverse Genetics (methods)</term><term>Virology (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Clones cellulaires</term><term>Coronavirus (génétique)</term><term>Génétique inverse ()</term><term>Réplicon</term><term>Virologie ()</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Genetics</term><term>Virology</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Clone Cells</term><term>Replicon</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clones cellulaires</term><term>Génétique inverse</term><term>Réplicon</term><term>Virologie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Highlights</title><p><list list-type="simple" id="lis0005"><list-item id="lsti0005"><label>•</label><p id="par0005">The four different reverse genetic systems developed for CoVs are reviewed and discussed.</p></list-item><list-item id="lsti0010"><label>•</label><p id="par0010">These systems were based on the use of BACs, in vitro ligation and vaccinia virus as a vector.</p></list-item><list-item id="lsti0015"><label>•</label><p id="par0015">CoV reverse genetics is an essential tool for the study of CoV replication, virus–host interaction and pathogenesis.</p></list-item><list-item id="lsti0020"><label>•</label><p id="par0020">These reverse genetic systems allow the development of genetically defined vaccines and the analysis of antiviral drugs.</p></list-item></list></p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Adedeji, A O" uniqKey="Adedeji A">A.O. 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Enjuanes</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Almazan, Fernando" sort="Almazan, Fernando" uniqKey="Almazan F" first="Fernando" last="Almazán">Fernando Almazán</name><name sortKey="Becares, Martina" sort="Becares, Martina" uniqKey="Becares M" first="Martina" last="Becares">Martina Becares</name><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name><name sortKey="Marquez Jurado, Silvia" sort="Marquez Jurado, Silvia" uniqKey="Marquez Jurado S" first="Silvia" last="Marquez-Jurado">Silvia Marquez-Jurado</name><name sortKey="Morales, Lucia" sort="Morales, Lucia" uniqKey="Morales L" first="Lucia" last="Morales">Lucia Morales</name><name sortKey="Sola, Isabel" sort="Sola, Isabel" uniqKey="Sola I" first="Isabel" last="Sola">Isabel Sola</name><name sortKey="Zu Iga, Sonia" sort="Zu Iga, Sonia" uniqKey="Zu Iga S" first="Sonia" last="Zu Iga">Sonia Zu Iga</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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